final concentration meaning in English
最后浓度
最终浓度
Examples
- Expression was induced by adding iptg to a final concentration of imm and incubation with shaking at 30 for 3 h
以2 %比例接种入新鲜mg培养基，培养至对数中期，加入iptg至终浓度为1mm ，在温度为30的条件下诱导表达3小时。 - The different bivalent cations with the same final concentration were put into the dnaase reacting system for 15 minute at ph5 . 4 , at 41 , then dnaase ' s activity unit was detected
在dna酶的酶促反应体系中加入终浓度相同的二价金属阳离子41下、 phs 4准确反应15分钟后测定酶的活力单位。 - The optimum precipitation conditions of jujube date polysaccharide were obtained as follows : the extracting liquid was concentrated 4 times , adding 4 times volume ethanol and making the final concentration of alcohol 80 %
摘要本文研究了红枣多糖的沉淀条件，并对所提取的红枣多糖粗品的抗氧化作用进行了探讨。 - Medium experiments were arranged under uniform design , and then an optimum medium was got accordingly . the culture liquid was centrifugalized at 3 , 500r / min for 30min , then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others
通过硫酸铵分级沉淀、 deaesephadexa - 50阴离子交换凝胶层析和sephadexg - 75凝胶柱层析对发酵液进行分离和纯化，并得到电泳纯的酶。 - Expression and purification of recombinant rtas the expression plasmid pkk223 . 3 - rta was introduced into e . coli jm 109 by cacl2 - mediated method . the cells were grown until an optical density at 600 nm reached 0 . 6 . expression was induced in the presence of iptg ( final concentration 1 mm ) and incubated at30 for 3h )
以mtt法分别测定纯化后的rta与rta一yqrl蛋白对体外培养的wish (人羊膜上皮细胞) 、 skov3 (人卵巢癌细胞) 、和hela (人宫颈癌细胞)的毒性作用。